JNK Antikörper

Produktnummer ABIN967329

Dieses Anti-JNK-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von JNK in WB und IP. Geeignet für Human.

Kurzübersicht für JNK Antikörper (ABIN967329)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JNK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: G151-333

Produktdetails anti-JNK Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human JNK1 fusion protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (0.25-1.0 µg/ml). Other applications not routinely tested include immunoprecipitation (1-2 µg/1x10e6 cells). HeLa control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Component No. 51-16516N, store lysate at -20°C). Additional control lysate (ABIN968535) is sold separately.

Kommentare:

Related Products: ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Details zu JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Andere Bezeichnung: JNK1

Hintergrund: C-Jun NH2-terminal kinase (JNK1) binds to the c-Jun terminal transactivation domain and phosphorylates it on Ser-63 and Ser-73. Phosphorylation enhances the transcriptional activity of c-Jun. The Ser-Pro-acidic sequence targeted by JNK1 kinase activity establishes it as a proline-directed kinase related to the MAP kinases and cyclin dependent kinases. JNK1 migrates with an apparent molecular weight of 46 kDa by SDS-PAGE. A related protein of 55 kDa has similar kinase activity. JNK1 may act as a tumor promoter in response to UV-irradiation since its activity is potently stimulated by such radiation. This has relevance to the observations that c-Jun transcriptional activity is upregulated by UV irradiation. The G151-333 antibody recognizes both the 46 kDa JNK1 and a related ~55 kDa protein (which may be a modified form of JNK1). It does not cross-react with JNK2. G151-333 precipitates an active kinase. A bacterially expressed fusion protein of human JNK1 was used as immunogen.

Molekulargewicht: 46 kDa

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine