TNF alpha Antikörper (AA 115-130)

Produktnummer ABIN967215

Der Maus Monoklonal anti-TNF alpha Antikörper (Klon B428) (ABIN967215) detektiert spezifisch TNF alpha in FACS und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNF alpha Antikörper (AA 115-130) (ABIN967215)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B428

Produktdetails anti-TNF alpha Antikörper

Bindungsspezifität: AA 115-130

Spezifität: This antibody reacts with TNFa.

Aufreinigung: Concentrated.

Immunogen: Synthetic TNF-alpha 115-130 hexadecapeptide linked to carrier protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100
ELISA 1:500 -1:1000 (this antibody is not purified and can not be used to coat ELISA plates) .
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: cytoplasmic and extracellular.
Recommended Positive Control: Prostate cancer

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor Alpha

Molekulargewicht: 17 kDa

Gen-ID: 7124

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome