NF-kB p65 Antikörper (C-Term)

Produktnummer ABIN964680

Hersteller Produkt- Nr.: 200-301-065

Dieses Anti-NF-kB p65-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NF-kB p65 in WB, IHC, ELISA, IF und FM. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für NF-kB p65 Antikörper (C-Term) (ABIN964680)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon: 27F9-G4

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-301-065

Hersteller: Rockland

Verwendungszweck: NFkB p65 Antibody

Kreuzreaktivität (Details): Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.

Produktmerkmale: Synonyms: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

Aufreinigung: Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody.

Sterilität: Sterile filtered

Immunogen:

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200-1:600

Application Note: Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:50,000-1:100,000

IF Microscopy Dilution: 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-NF-kB p65 Antikörper (ABIN964680)

Sikorski, Mehta, Inngjerdingen, Thakor, Kling, Kalina, Nyman, Stensland, Zhou, de Souza, Holden, Stuchly, Templin, Lund-Johansen: "A high-throughput pipeline for validation of antibodies." in: Nature methods, Vol. 15, Issue 11, pp. 909-912, (2019) (PubMed).

Merkhofer, Cogswell, Baldwin: "Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha." in: Oncogene, Vol. 29, Issue 8, pp. 1238-48, (2010) (PubMed).

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: RELA

Hintergrund: Background: NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research.

Gen-ID: 5970, 223468676

UniProt: Q04206

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine