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CD11b Antikörper

This anti-CD11b antibody (ABIN93956) is a Mouse Monoclonal antibody detecting CD11b in FACS, IP. Suitable for Human.
Produktnummer ABIN93956

Quick Overview for CD11b Antikörper (ABIN93956)

Target

Alle CD11b (ITGAM) Antikörper anzeigen
CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität

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Human

Wirt

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Maus

Klonalität

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Monoklonal

Konjugat

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Dieser CD11b Antikörper ist unkonjugiert

Applikation

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Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon

MEM-174
  • Verwendungszweck

    Anti-Hu CD11b Purified

    Spezifität

    The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

    Kreuzreaktivität (Details)

    Human

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Human granulocytes

    Isotyp

    IgG2a
  • Applikationshinweise

    Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes.
    Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants.
    Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 mL of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, (2015) (PubMed).

    Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

  • Target

    CD11b (ITGAM) (Integrin alpha M (ITGAM))

    Andere Bezeichnung

    CD11b

    Hintergrund

    Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

    Gen-ID

    3684

    UniProt

    P11215

    Pathways

    Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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