TNF alpha Antikörper

Produktnummer ABIN872451

Dieses Anti-TNF alpha-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TNF alpha in WB, IHC (p) und IF (p). Geeignet für Human, Schwein und Hund. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für TNF alpha Antikörper (ABIN872451)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human, Schwein, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-TNF alpha Antikörper

Kreuzreaktivität: Hund, Human, Schwein

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from dog TNF-alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB (1:100-500),IHC-P (1:100-500),IF (p) (1:100-500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Referenzen für anti-TNF alpha Antikörper (ABIN872451)

Zhang, Liu, Hu, Jiang, Zhu: ""Proof-of-principle" concept for ultrasensitive detection of cytokines based on the electrically heated carbon paste electrode." in: Chemical communications (Cambridge, England), Vol. 47, Issue 23, pp. 6551-3, (2011) (PubMed).

Liao, Zhou, Ji, Zhang: "Eccentric contraction induces inflammatory responses in rat skeletal muscle: role of tumor necrosis factor-alpha." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 298, Issue 3, pp. R599-607, (2010) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF-alpha

Hintergrund:

Synonyms: APC1, Cachectin, D, Dferentiation inducing factor, Macrophage cytotoxic factor, MCF, Necrosin, TNF a, TNF alpha, TNF, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor, Tumour Necrosis Factor Alpha, TNFA_HUMAN.

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Molekulargewicht: 17/26kDa

Gen-ID: 403922

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome