NRF2 Antikörper (AA 445-458)

Produktnummer ABIN768601

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch NRF2 in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NRF2 Antikörper (AA 445-458) (ABIN768601)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NRF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-NRF2 Antikörper

Bindungsspezifität: AA 445-458

Verwendungszweck: NRF2 (aa445-458)

Sequenz: KHSSRLEAHL TRDE

Spezifität: This antibody is expected to recognize all reported isoforms (

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KHSSRLEAHLTRDE, from the internal region of the protein sequence according to NP_006155.2, NP_001138884.1, NP_001138885.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in Human Lung lysates and approx 75 kDa in preliminary testing of Mouse and Rat Liver and KNRK, MDCK, and nuclear NIH3T3 cell lysate (calculated MW of 67.8 kDa according to NP_006155.2). This molecular weight rate

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Andere Bezeichnung: NFE2L2

Hintergrund: NFE2L2, nuclear factor (erythroid-derived 2)-like 2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2, OTTHUMP00000205251, OTTHUMP00000207980, nuclear factor erythroid 2-related factor 2, nuclear factor, erythroid derived 2, like 2

Molekulargewicht: 67.8kDa according to NP_006155.2

Gen-ID: 4780, 18024, 83619

NCBI Accession: NP_006155, NP_001138884, NP_001138885

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling