p21 Antikörper (pThr57) (Cy5)

Produktnummer ABIN756881

Der Kaninchen Polyklonal anti-p21 Antikörper wird verwendet zum Nachweis von p21 in Proben von Human. Er wurde validiert für WB, IF (p) und IF (cc).

Kurzübersicht für p21 Antikörper (pThr57) (Cy5) (ABIN756881)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p21 Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Produktdetails anti-p21 Antikörper (Cy5)

Bindungsspezifität: pThr57

Verwendungszweck: phospho-CDKN1A/p21 (Thr57) Polyclonal Antibody, Cy5 Conjugated

Spezifität: This phosphorylation site is homologous to Thr56 in Mouse.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr57 [TE(p-T)PL]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: CDKN1A

Hintergrund:

Synonyms: P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, CDKN1A, MDA6, PIC1

Background: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Gen-ID: 1026

UniProt: P38936

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie