NRF2 Antikörper (AA 67-601)

Produktnummer ABIN7876383

Der Kaninchen Polyklonal anti-NRF2 Antikörper wird verwendet zum Nachweis von NRF2 in Proben von Human. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für NRF2 Antikörper (AA 67-601) (ABIN7876383)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NRF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NRF2 Antikörper

Bindungsspezifität: AA 67-601

Verwendungszweck: NRF2 Antibody / Nuclear factor erythroid 2-related factor 2 / NFE2L2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NRF2/NFE2L2 recombinant protein (Position: E67-D601) was used as the immunogen for the NRF2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NRF2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NRF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Andere Bezeichnung: NRF2

Hintergrund: NRF2 antibody detects Nuclear factor erythroid 2-related factor 2, a transcription factor that regulates antioxidant defense and cellular detoxification. The UniProt recommended name is Nuclear factor erythroid 2-related factor 2 (NFE2L2). This basic leucine zipper (bZIP) transcription factor activates expression of antioxidant response element (ARE)-driven genes that protect cells against oxidative and electrophilic stress.

Functionally, NRF2 antibody identifies a 605-amino-acid protein that binds to DNA as a heterodimer with small Maf proteins. Under basal conditions, NRF2 is bound by KEAP1 in the cytoplasm and targeted for proteasomal degradation. Upon oxidative stress, NRF2 escapes KEAP1 repression, translocates to the nucleus, and induces genes encoding detoxification enzymes, antioxidant proteins, and drug-metabolizing enzymes such as NQO1, HO-1, and GCLM.

The NFE2L2 gene is located on chromosome 2q31.2 and is expressed in multiple tissues, with high levels in liver, lung, and kidney. NRF2 acts as a master regulator of cellular defense mechanisms, maintaining redox balance, xenobiotic metabolism, and mitochondrial function.

Pathologically, persistent NRF2 activation contributes to tumorigenesis and drug resistance, while impaired NRF2 signaling increases susceptibility to oxidative injury and chronic diseases such as neurodegeneration and fibrosis. Research using NRF2 antibody supports studies in transcriptional regulation, redox signaling, and cytoprotection.

NRF2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect transcription factors involved in antioxidant defense. NSJ Bioreagents provides NRF2 antibody reagents optimized for oxidative stress, cancer, and metabolism research.

Structurally, Nuclear factor erythroid 2-related factor 2 contains multiple Neh (Nrf2-ECH homology) domains that mediate DNA binding, KEAP1 interaction, and transcriptional activation. This antibody aids investigation of NRF2's dynamic regulation and its role in cellular adaptation to stress.

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling