IRF7 Antikörper (pSer471, pSer472)

Produktnummer ABIN742703

Der Kaninchen Polyklonal Anti-IRF7-Antikörper wurde für WB, ELISA, FACS und IHC (p) validiert. Er ist geeignet, IRF7 in Proben von Human, Maus und Ratte zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für IRF7 Antikörper (pSer471, pSer472) (ABIN742703)

Target: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IRF7 Antikörper

Bindungsspezifität: pSer471, pSer472

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-IRF7 Antikörper (ABIN742703)

Le Bel, Gosselin: "Leukotriene B4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139856, (2015) (PubMed).

Han, Choi, Uyangaa, Kim, Kim, Kim, Kim, Eo: "Distinct dictation of Japanese encephalitis virus-induced neuroinflammation and lethality via triggering TLR3 and TLR4 signal pathways." in: PLoS pathogens, Vol. 10, Issue 9, pp. e1004319, (2014) (PubMed).

Menasria, Boivin, Lebel, Piret, Gosselin, Boivin: "Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection." in: Journal of virology, Vol. 87, Issue 13, pp. 7301-8, (2013) (PubMed).

Details zu IRF7

Target: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Andere Bezeichnung: IRF7 +

Hintergrund:

Synonyms: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7, IRF7

Background: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.

Gen-ID: 3665

UniProt: Q92985

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagie