TLR4 Antikörper (CF®740)

Produktnummer ABIN7660907

Der Maus Monoklonal Anti-TLR4-Antikörper wurde für validiert. Er ist geeignet, TLR4 in Proben von Human, Ratte, Hund, Schwein, Meerschweinchen und Affe zu detektieren.

Kurzübersicht für TLR4 Antikörper (CF®740) (ABIN7660907)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Human, Ratte, Hund, Schwein, Meerschweinchen, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR4 Antikörper ist konjugiert mit CF®740

Applikation: Bitte anfragen

Klon: TLR4-230

Produktdetails anti-TLR4 Antikörper (CF®740)

Verwendungszweck: CD284 / TLR4(TLR4/230), CF740 conjugate

Produktmerkmale: This MAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TLR4 protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HeLa, PC12, human peripheral blood cells. Dendritic cells, macrophage and monocytes in tonsil, placenta and adrenal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: CD284

Hintergrund:

Synonyms: ARMD10, CD284, hToll, TLR4, TOLL, Toll Endotoxin Hyporesponsiveness, Toll-like receptor 4

Gene Symbol: TLR4

Molekulargewicht: 95-120 kDa

Gen-ID: 7099

UniProt: O00206

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine