SREBF1 Antikörper (CF®740)

Produktnummer ABIN7660428

Dieses Anti-SREBF1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SREBF1 in . Geeignet für Human.

Kurzübersicht für SREBF1 Antikörper (CF®740) (ABIN7660428)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SREBF1 Antikörper ist konjugiert mit CF®740

Applikation: Bitte anfragen

Klon: SREBP1-1578

Produktdetails anti-SREBF1 Antikörper (CF®740)

Verwendungszweck: SREBP1(SREBP1/1578), CF740 conjugate

Produktmerkmale: The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix-leucine zipper (bHLH-zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SREBF1 protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Ubiqutious expression in adult and fetal tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Andere Bezeichnung: SREBP1

Hintergrund:

Synonyms: ADD 1, bHLHd1, Class D basic helix-loop-helix protein 1, Processed sterol regulatory element-binding protein 1, SREBF1, SREBP1c, SREBP-1, Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1, Sterol regulatory element binding transcription factor 1, Sterol regulatory element-binding transcription factor 1

Gene Symbol: SREBF1

Molekulargewicht: 68/125 kDa mature/precursor

Gen-ID: 6720

UniProt: P36956

Pathways: AMPK Signaling, Caspase Kaskade in der Apoptose, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha