CD36 Antikörper (CF®647)

Produktnummer ABIN7651307

Der Maus Monoklonal Anti-CD36-Antikörper wurde für validiert. Er ist geeignet, CD36 in Proben von Human zu detektieren.

Kurzübersicht für CD36 Antikörper (CF®647) (ABIN7651307)

Target: CD36

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD36 Antikörper ist konjugiert mit CF®647

Applikation: Bitte anfragen

Klon: GPIIIb-1654

Produktdetails anti-CD36 Antikörper (CF®647)

Verwendungszweck: CD36 (Platelet & Microvessel Marker)(GPIIIb/1654), CF647 conjugate

Produktmerkmale: Recognizes a protein of 80 kDa-90 kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4 % of Japanese and East Asia population lack CD36. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GPIIIb protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HEL or U937 cells. Platelets, monocytes, macrophages, microvascular endothelial cells in a tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD36

Target: CD36

Andere Bezeichnung: CD36

Hintergrund:

Synonyms: Adipocyte membrane protein, CHDS7, Collagen receptor, platelet, fatty acid translocase (FAT), Fatty acid transport protein, glycoprotein iiib (GP IIIb), PAS IV, PAS-4, pas4 protein, platelet collagen receptor, Platelet glycoprotein 4, scarb3, Scavenger receptor class B member 3, thrombospondin receptor

Gene Symbol: CD36

Tissue Expression: Platelets|Erythrocyte precursors|Mammary gland|Microvascular endothelial cells|Monocytes/macrophages

Molekulargewicht: 80-90 kDa

Gen-ID: 948

UniProt: P16671

Pathways: TLR Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteine