JAK2 Antikörper (Cytoplasmic Domain, Cytosolic)

Produktnummer ABIN7602956

Dieses Anti-JAK2-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von JAK2 in WB, IHC, IF, ICC und IP. Geeignet für Human, Ratte und Maus.

Kurzübersicht für JAK2 Antikörper (Cytoplasmic Domain, Cytosolic) (ABIN7602956)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser JAK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: COG-10

Produktdetails anti-JAK2 Antikörper

Bindungsspezifität: Cytoplasmic Domain, Cytosolic

Verwendungszweck: Anti-JAK2 Monoclonal Antibody

Produktmerkmale: Anti-JAK2 Monoclonal Antibody (ABIN7602956). Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50-100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Konzentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Details zu JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Andere Bezeichnung: JAK2

Hintergrund:

Synonyms: Signal transducer and activator of transcription 3,Acute-phase response factor,STAT3,APRF,

Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Molekulargewicht: 131 kDa

UniProt: O60674

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response