NRF2 Antikörper (AA 34-605)

Produktnummer ABIN7601400

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NRF2 in WB, ELISA, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NRF2 Antikörper (AA 34-605) (ABIN7601400)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NRF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NRF2 Antikörper

Bindungsspezifität: AA 34-605

Verwendungszweck: Anti-NFE2L2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NFE2L2 Antibody Picoband® (ABIN7601400). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NFE2L2 recombinant protein (Position: R34-N605).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chan, J. Y., Cheung, M.-C., Moi, P., Chan, K., Kan, Y. W. Chromosomal localization of the human NF-E2 family of bZIP transcription factors by fluorescence in situ hybridization. Hum. Genet. 95: 265-269, 1995. 2. He, C. H., Gong, P., Hu, B., Stewart, D., Choi, M. E., Choi, A. M. K., Alam, J. Identification of activating transcription factor 4 (ATF4) as an Nrf2-interacting protein: implication for heme oxygenase-1 gene regulation. J. Biol. Chem. 276: 20858-20865, 2001. 3. Lee, J.-M., Chan, K., Kan, Y. W., Johnson, J. A. Targeted disruption of Nrf2 causes regenerative immune-mediated hemolytic anemia.Proc. Nat. Acad. Sci. 101: 9751-9756, 2004.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Andere Bezeichnung: NFE2L2

Hintergrund:

Synonyms: Interleukin-13,IL-13,IL13,NC30,

Tissue Specificity: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Background: NFE2L2 (nuclear factor (erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-ed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas (G12D) and BRaf (V619E), and in human pancreatic cancer.

Molekulargewicht: 100 kDa

Gen-ID: 4780

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling