IL4 Receptor Antikörper (AA 26-719)

Produktnummer ABIN7600970

Dieses Anti-IL4 Receptor-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IL4 Receptor in ELISA, IHC und FACS. Geeignet für Maus und Ratte.

Kurzübersicht für IL4 Receptor Antikörper (AA 26-719) (ABIN7600970)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL4 Receptor Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-IL4 Receptor Antikörper

Bindungsspezifität: AA 26-719

Verwendungszweck: Anti-IL4R Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-IL4R Antibody Picoband® (ABIN7600970). Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived rat IL4R recombinant protein (Position: I26-E719).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL,
1. Bergin, A.-M., Balder, B., Kishore, S., Sward, K., Hahn-Zoric, M., Lowhagen, O., Hanson, L. A., Padyukov, L. Common variations in the IL4R gene affect splicing and influence natural expression of the soluble isoform. Hum. Mutat. 27: 990-998, 2006. 2. Blaeser, F., Bryce, P. J., Ho, N., Raman, V., Dedeoglu, F., Donaldson, D. D., Geha, R. S., Oettgen, H. C., Chatila, T. A. Targeted inactivation of the IL-4 receptor alpha chain I4R motif promotes allergic airway inflammation. J. Exp. Med. 198: 1189-1200, 2003. 3. Ikizawa, K, Yanagihara Y (Feb. 2000). "Possible involvement of Shc in IL-4-induced germline epsilon transcription in a human B cell line". Biochem. Biophys. Res.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: Il4r

Hintergrund:

Synonyms: Oncostatin-M, OSM, Osm

Tissue Specificity: Widely expressed. Expressed at higher levels in liver, skin and spleen.

Background: IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Molekulargewicht: 180 kDa

Gen-ID: 25084

UniProt: Q63257

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process