MEK2 Antikörper (AA 1-400)

Produktnummer ABIN7599356

Der Maus Monoklonal Anti-MEK2-Antikörper wurde für WB, IHC, IF, FACS und ICC validiert. Er ist geeignet, MEK2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MEK2 Antikörper (AA 1-400) (ABIN7599356)

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 2H4

Produktdetails anti-MEK2 Antikörper

Bindungsspezifität: AA 1-400

Verwendungszweck: Anti-MEK2/MAP2K2 Antibody Picoband® (monoclonal, 2H4)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MEK2/MAP2K2 Antibody Picoband® (monoclonal, 2H4) (ABIN7599356). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MEK2/MAP2K2 recombinant protein (Position: M1-V400).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1.""Entrez Gene: MAP2K2 mitogen-activated protein kinase kinase 2" 2. Zheng, C. F., Guan, K. L. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 268: 11435-11439, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Andere Bezeichnung: MAP2K2

Hintergrund:

Synonyms: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX

Tissue Specificity: Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

Background: Dual specificity mitogen-activated protein kinase kinase 2 (MAP2K2), also called PRKMK2 or MEK2, is an enzyme that in humans is encoded by the MAP2K2 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MAP2K2 is mapped to 19p13.3. This kinase is known to play a critical role in mitogen growth factor signal transduction, and the inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. Recombinant MEK2 and MEK1 both could activate human ERK1 in vitro, and they further characterized biochemically the 2 MAP2Ks. MAP2K2 has been shown to interact with MAPK3 and ARAF.

Molekulargewicht: 45 kDa

Gen-ID: 5605

UniProt: P36507

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling