Collagen IV Antikörper

Produktnummer ABIN7565969

Der Kaninchen Polyklonal Anti-Collagen IV-Antikörper wurde für WB, ELISA, IHC, IP, DB und FM validiert. Er ist geeignet, Collagen IV in Proben von Säugetier zu detektieren.

Kurzübersicht für Collagen IV Antikörper (ABIN7565969)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-Collagen IV Antikörper

Verwendungszweck: Collagen Type IV Antibody

Kreuzreaktivität (Details): Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Produktmerkmale: Synonyms: rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1

Aufreinigung: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Collagen Type IV from human and bovine placenta

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis. Immunohistochemistry Dilution: 1:50 - 1:200 Western Blot Dilution: 1:1,000 - 1:10,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:50,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.18 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV

Hintergrund: Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Gen-ID: 1282

NCBI Accession: NP_001290039

UniProt: P02462