GATA4 Antikörper

Produktnummer ABIN7565766

Dieses Anti-GATA4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GATA4 in WB und ELISA. Geeignet für Human.

Kurzübersicht für GATA4 Antikörper (ABIN7565766)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GATA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 10D8-B10-D4

Produktdetails anti-GATA4 Antikörper

Verwendungszweck: GATA4 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93 % homology.

Produktmerkmale: Synonyms: mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody

Aufreinigung: Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-GATA4 antibody has been tested by ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500-1:1000 ELISA Dilution: 1:2000-1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Andere Bezeichnung: GATA4

Hintergrund: Background: GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gen-ID: 2626

NCBI Accession: NP_002043

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis