TNF alpha Antikörper (AA 86-150) (Cy3)

Produktnummer ABIN724583

Der Kaninchen Polyklonal anti-TNF alpha Antikörper wird verwendet zum Nachweis von TNF alpha in Proben von Human, Maus, Ratte, Kaninchen und Schwein. Er wurde validiert für WB, IF (p) und IF (cc).

Kurzübersicht für TNF alpha Antikörper (AA 86-150) (Cy3) (ABIN724583)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human, Maus, Ratte, Kaninchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist konjugiert mit Cy3

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Produktdetails anti-TNF alpha Antikörper (Cy3)

Bindungsspezifität: AA 86-150

Kreuzreaktivität: Human, Maus, Schwein, Kaninchen, Ratte

Homologie: Dog,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TNF alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF alpha

Hintergrund:

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF

Background: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Molekulargewicht: 17/26kDa

Gen-ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome