Rekombinanter CD40 Antikörper (Extracellular Domain)

Produktnummer ABIN7543234

Dieses Anti-CD40-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD40 in FACS, ELISA, IP, IF und Agon. Geeignet für Maus.

Kurzübersicht für Rekombinanter CD40 Antikörper (Extracellular Domain) (ABIN7543234)

Target: CD40

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD40 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Agonist (Agon)

Klon: 1C10

Produktdetails anti-CD40 Antikörper

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Anti-CD40 [1C10], Rabbit IgG, kappa

Spezifität: This antibody binds mouse CD40, which is a member of the tumor necrosis factor receptor superfamily. CD40 is an integral membrane protein found on the surface of B lymphocytes.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing a rat with 20 mug of soluble extracellular domain of recombinant murine CD40 (sCD40).

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody was reported to directly induce a specific proliferative response from mature murine B cells, up-regulate several B cell surface antigens, and rescue immature B lymphoma cells from anti-IgM-induced growth arrest. This initial screening of hybridoma supernatants for reactivity towards CD40 transfected L cells was done using flow cytometry. This antibody recognized recombinant CD40 expressed on L cells and the soluble extracellular domain of murine CD40 coated onto microtiter plates in an ELISA. This antibody was also used in the immunofluorescent staining of adult murine spleen cells. The staining was restricted to the B220+ population. This antibody also specifically immunoprecipitated a 45- kDa protein from the surface of purified murine splenic B cells (PMID: 7519997). This antibody was capable of blocking binding of FGK45 to CD40 on murine B cells in vitro (PMID: 24416732). The mouse IgG1 version of this antibody had a tenfold higher affinity for mouse FcγRIIB as compared to mouse IgG2a or the parental rat IgG2b (PMID: 21852502). The mouse IgG1 version of this antibody was used in the treatment of M38 tumor bearing mice and it was reported that there was a strong increase in PD-L1 expression both on tumor-infiltrating monocytes and TAMs (PMID: 25623164).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund: TNFRSF5, B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, MGC9013, T-cell differentiation antigen, Tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

UniProt: P27512

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints