SREBF1 Antikörper (N-Term)

Produktnummer ABIN7540628

Hersteller Produkt- Nr.: 600-401-mn1s

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SREBF1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SREBF1 Antikörper (N-Term) (ABIN7540628)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SREBF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SREBF1 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 600-401-mn1s

Hersteller: Rockland

Verwendungszweck: SREBP1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human SREBP1.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-SREBP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human SREBP1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 5 μg/mL

Kommentare:

Anti-SREBP1 Antibody has been tested in ELISA and WB.  Expect reactivity with different human isoforms and a band at ~124.6, ~121.7, ~113.6, ~48.3kDa in western blot using appropriate tissue or lysates.  Positive control used: HeLa and A549 lysates in Western Blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Andere Bezeichnung: SREBF1

Hintergrund: Rabbit Anti-SREBP1 Antibody, Rabbit Anti-Sterol Regulatory Element Binding Transcription Factor 1 Antibody, Class D Basic Helix-Loop-Helix Protein 1, SREBP1, BHLHd1, Sterol Regulatory Element-Binding Transcription Factor 1, Sterol Regulatory Element-Binding Protein 1, SREBP-1, BHLHD1,SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1) is a transcriptional activator required for lipid homeostasis. It regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. It binds to the sterol regulatory element 1 (SRE-1) (5-ATCACCCCAC-3), which is a motif that is found in the promoter of the low-density lipoprotein receptor gene and other genes involved in sterol biosynthesis. SREBP1 has dual sequence specificity binding to both an E-box motif (5-ATCACGTGA-3) and to SRE-1 (5-ATCACCCCAC-3). Anti-SREBP1 Antibody is useful for researchers interested in Fatty Liver Disease, Smith-Magenis Syndrome, Adipogenesis, DNA binding transcription factor activity and chromatin binding.

Gen-ID: 6720

NCBI Accession: NP_001005291

UniProt: P36956

Pathways: AMPK Signaling, Caspase Kaskade in der Apoptose, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha