Rekombinanter p21 Antikörper (AA 120-138)

Produktnummer ABIN7487749

Der Kaninchen Chimeric anti-p21 Antikörper (Klon Y13-238) (ABIN7487749) detektiert spezifisch p21 in IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Rekombinanter p21 Antikörper (AA 120-138) (ABIN7487749)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP)

Klon: Y13-238

Produktdetails anti-p21 Antikörper

Bindungsspezifität: AA 120-138

Verwendungszweck: Anti-p21 [Y13-238], Rabbit IgG, lambda

Spezifität:

The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21's but not v-K-ras or rodent c-K-ras p21's. This antibody also recognizes human c-H-ras and c-K-ras p21's, but not human c-N-ras.

Application Notes original Clone: Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti-ras non-neutralising scFv (Cochet 1998).

Kreuzreaktivität: Maus

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.

Isotyp: IgG, lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Rat

OriginalFormat: IgG2a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: p21

Hintergrund: Ras, p21ras,

UniProt: P78460, O70564

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie