Rekombinanter Collagen IV Antikörper

Produktnummer ABIN7477425

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch Collagen IV in IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Collagen IV Antikörper (ABIN7477425)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-704R

Produktdetails anti-Collagen IV Antikörper

Immunogen: Purified human placental extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.

Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen IV

Hintergrund: Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR,Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02462, P08572, P29400, P53420