RAD50 Antikörper (N-Term)

Produktnummer ABIN7468850

Dieses Anti-RAD50-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RAD50 in WB, IP und IHC (p). Geeignet für Human.

Kurzübersicht für RAD50 Antikörper (N-Term) (ABIN7468850)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: KO Validated

Produktdetails anti-RAD50 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Rad50. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro2A , GL261 , NT2D1 , HeLa nuclear extract , PC-12 , HeLa Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.78 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Andere Bezeichnung: RAD50 double strand break repair protein

Hintergrund:

Synonyms: RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]

Molekulargewicht: 154 kDa

Gen-ID: 10111

UniProt: Q92878

Pathways: DNA Reparatur, Protein targeting to Nucleus