Caspase 8 Antikörper

Produktnummer ABIN7464822

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Caspase 8 in WB, ICC, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Caspase 8 Antikörper (ABIN7464822)

Target: Caspase 8 (CASP8)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: KO Validated

Produktdetails anti-Caspase 8 Antikörper

Kreuzreaktivität: Cat, Hund, Human, Maus, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Caspase 8. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.74 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: caspase 8

Hintergrund: Caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5,This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]

Molekulargewicht: 55 kDa

Gen-ID: 841

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades