SMAD4 Antikörper (C-Term)

Produktnummer ABIN7464820

Der Kaninchen Polyklonal Anti-SMAD4-Antikörper wurde für WB, IF, ICC und IHC (p) validiert. Er ist geeignet, SMAD4 in Proben von Human zu detektieren.

Kurzübersicht für SMAD4 Antikörper (C-Term) (ABIN7464820)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SMAD4 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2 , DDDDK-tagged SMAD4-transfected 293T

Validation: Comparison, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.58 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD family member 4

Hintergrund: SMAD family member 4 , DPC4 , JIP , MADH4 , MYHRS,This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq]

Molekulargewicht: 60 kDa

Gen-ID: 4089

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie