BAD Antikörper (AA 1-50)

Produktnummer ABIN7453524

Der Kaninchen Polyklonal anti-BAD Antikörper (ABIN7453524) detektiert spezifisch BAD in WB, IP und IHC (fp). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für BAD Antikörper (AA 1-50) (ABIN7453524)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-BAD Antikörper

Bindungsspezifität: AA 1-50

Verwendungszweck: Rabbit anti-BAD Antibody, Affinity Purified

Homologie: Rat

Aufreinigung: Affinity Purified

Immunogen: between AA 1 and 50

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 10 μg/mg lysate

WB: Not recommended. ABIN7453524 has not performed satisfactorily when used for WB of BAD in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BAD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Andere Bezeichnung: BAD

Hintergrund: Background: BAD (Bcl2 agonist of cell death) is a member of the Bcl-2 family. Bcl-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein [taken from NCBI Entrez Gene (GeneID:572)].

Gen-ID: 572

NCBI Accession: NP_004313

UniProt: Q92934

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, Apoptose, Fc-epsilon Rezeptor Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events