MEK2 Antikörper (pThr394)

Produktnummer ABIN7213036

Der Kaninchen Polyklonal Anti-MEK2 Antikörper wurde für WB, ELISA, IP und IHC (p) validiert. Er ist geeignet, MEK2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MEK2 Antikörper (pThr394) (ABIN7213036)

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MEK2 Antikörper

Bindungsspezifität: pThr394

Verwendungszweck: MEK-2 (phospho Thr394) Polyclonal Antibody

Spezifität: Phospho-MEK-2 (T394) Polyclonal Antibody detects endogenous levels of MEK-2 protein only when phosphorylated at T394.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MEK-2 Phospho-Thr394

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 μg/mg lysate), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Andere Bezeichnung: MEK-2

Hintergrund: Rabbit Anti-MEK-2 (phospho Thr394) Polyclonal Antibody,MAP2K2, MEK2, MKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2,Mitogen-activated protein kinase 3 encoded by MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. Mitogen-activated protein kinase 3 is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. Mitogen-activated protein kinase 3 can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of Mitogen-activated protein kinase 3 is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for MAP2K3.,Dual specificity mitogen-activated protein kinase kinase 2

Gen-ID: 5606

UniProt: P36507

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling