JNK Antikörper (pThr183)

Produktnummer ABIN7213024

Der Kaninchen Polyklonal anti-JNK Antikörper wird verwendet zum Nachweis von JNK in Proben von Human, Maus, Ratte, Huhn, Schwein und Fisch. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für JNK Antikörper (pThr183) (ABIN7213024)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reaktivität: Human, Maus, Ratte, Huhn, Schwein, Fisch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JNK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-JNK Antikörper

Bindungsspezifität: pThr183

Verwendungszweck: Rabbit Anti-JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody

Spezifität: Phospho-JNK1/2/3 (T183/Y185) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183/Y185.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human JNK1/2/3 Phospho-Thr183/Y185

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-2000,IHC 1:50-300,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Andere Bezeichnung: JNK1/2/3

Hintergrund: MAPK8, JNK1, PRKM8, SAPK1, SAPK1C, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK9, JNK2, PRKM9, SAPK1A, MiMitogen-activated protein kinase 8 encoded by MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Mitogen-activated protein kinase 8 is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. Mitogen-activated protein kinase 8 is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of MAPK8 suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Molekulargewicht: 46kD,54kD

Gen-ID: 5599, 5601, 5602

UniProt: P45983, P45984, P53779

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine