TNF alpha Antikörper

Produktnummer ABIN7140874

Dieses Anti-TNF alpha-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TNF alpha in ELISA. Geeignet für Rind (Kuh).

Kurzübersicht für TNF alpha Antikörper (ABIN7140874)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: ELISA

Klon: 2E1A5

Produktdetails anti-TNF alpha Antikörper

Kreuzreaktivität: Rind (Kuh)

Aufreinigung: Protein G purified

Immunogen: Recombinant Bovine TNF alpha protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF

Hintergrund:

Background: Tumor necrosis factor alpha (TNFα,also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNFα is produced by a wide variety of immune and epithelial cell types. Bovine TNFα consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD). Within the ECD, bovine TNFα shares 64 % -83 % sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNFα.The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNFα by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNFα. TNFα trimers bind the ubiquitous TNF RI and the hematopoietic cell restricted TNF RII, both of which are also expressed as homotrimers. TNFα regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNFα is a key cytokine in several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

Aliases: Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2

UniProt: Q06599

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome