PIK3R2 Antikörper (AA 520-720)

Produktnummer ABIN7126793

Dieser Maus Monoklonal Antikörper detektiert spezifisch PIK3R2 in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PIK3R2 Antikörper (AA 520-720) (ABIN7126793)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PIK3R2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: PIK3R2-292

Produktdetails anti-PIK3R2 Antikörper

Bindungsspezifität: AA 520-720

Verwendungszweck: PIK3R2 / Phosphoinositide-3-kinase regulatory subunit 2 Antibody

Spezifität: Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85°™ and p85 æ), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110°™ and p110 æ interact with p85°™, and p110°™ has also been shown to interact with p85 æ in vitro. p110 79oΩL expression is restricted to white blood cells. It has been shown to bind p85°™ and p85 æ, but it apparently does not phosphorylate these subunits. p110 79oΩL seems to have the capacity to autophosphorylate. p110Á does not interact with the p85 subunits. It has been shown to be activated by °™ and æÁ heterotrimeric G proteins.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human PIK3R2 protein (around aa 520-720) (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human spleen or bladder.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Andere Bezeichnung: PIK3R2

Hintergrund:

p85 beta, P85B, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta), Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta), PI3 kinase p85 beta subunit, PI3K regulatory subunit beta, PIK3R2, PtdIns-3-kinase regulatory subunit p85-beta

Human spleen or bladder.

Molekulargewicht: 85kDa

Gen-ID: 5296, 371344

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling