BRAF Antikörper (AA 457-717)

Produktnummer ABIN7427177

Der Maus Monoklonal anti-BRAF Antikörper (Klon C5) (ABIN7427177) detektiert spezifisch BRAF in WB, IHC, ICC und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für BRAF Antikörper (AA 457-717) (ABIN7427177)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BRAF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: C5

Produktdetails anti-BRAF Antikörper

Bindungsspezifität: AA 457-717

Verwendungszweck: Monoclonal Antibody to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)

Spezifität: The antibody is a mouse monoclonal antibody raised against BRAF. It has been selected for its ability to recognize BRAF in immunohistochemical staining and western blotting.

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: Recombinant B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) corresdonding to Ile457~Leu717 with N-terminal His Tag

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.47 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Andere Bezeichnung: BRAF

Hintergrund: B-raf 1, BRAF1, RAFB1, V-Raf Murine Sarcoma Viral Oncogene Homolog B1

UniProt: P15056

Pathways: MAPK Signalweg, RTK Signalweg, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagie