TNF alpha Antikörper (AA 77-197)

Produktnummer ABIN7426703

Der Maus Monoklonal anti-TNF alpha Antikörper (Klon 1-1#) (ABIN7426703) detektiert spezifisch TNF alpha in WB, IHC, ICC und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Rhesusaffen.

Kurzübersicht für TNF alpha Antikörper (AA 77-197) (ABIN7426703)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 1-1#

Produktdetails anti-TNF alpha Antikörper

Bindungsspezifität: AA 77-197

Verwendungszweck: Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Spezifität: The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Val77~Gly197 Plus RGLSAGERVIDSALRSICPTISTLPSSGQV with N-terminal His Tag

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL,
Immunohistochemistry: 5-20 μg/mL,
Immunocytochemistry: 5-20 μg/mL,
Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNFa

Hintergrund: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

UniProt: P48094

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome