DEPTOR Antikörper (AA 220-270)

Produktnummer ABIN6992003

Der Kaninchen Polyklonal Anti-DEPTOR-Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, DEPTOR in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für DEPTOR Antikörper (AA 220-270) (ABIN6992003)

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DEPTOR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DEPTOR Antikörper

Bindungsspezifität: AA 220-270

Spezifität: DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist, this antibody will detect all three isoforms.

Aufreinigung: DEPTOR antibody is affinity chromatography purified via peptide column.

Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: DEPTOR Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Andere Bezeichnung: DEPTO

Hintergrund: DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).

Molekulargewicht: Predicted: 45 kDa

Observed: 42 kDa

Gen-ID: 64798

NCBI Accession: NP_073620

UniProt: Q8TB45

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Regulation of Cell Size, BCR Signaling, Warburg Effekt