PAK2 Antikörper (N-Term)

Produktnummer ABIN6990664

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PAK2 in WB, ELISA, IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PAK2 Antikörper (N-Term) (ABIN6990664)

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-PAK2 Antikörper

Bindungsspezifität: AA 20-70, N-Term

Aufreinigung: PAK2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PAK2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PAK2. The immunogen is located within amino acids 20-70 of PAK2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 0.5-2 μ,g/mL, IHC: 10 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PAK2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PAK2

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Andere Bezeichnung: PAK2

Hintergrund: PAK2 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.

Molekulargewicht: Predicted: 58kD

Observed: 58 kD kDa

Gen-ID: 5062

NCBI Accession: NP_002568

UniProt: Q13177

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Regulation of Lipid Metabolism by PPARalpha