PAK2 Antikörper (N-Term)

Produktnummer ABIN500441

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-PAK2 Antikörper wird verwendet zum Nachweis von PAK2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für PAK2 Antikörper (N-Term) (ABIN500441)

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PAK2 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects PAK2 at N-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Peptide affinity chromatography

Immunogen: 14 amino acid peptide from near the amino terminus of human PAK2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-PAK2 Antikörper (ABIN500441)

Choi, Pease, Chen, Zhang, Phee: "P21-activated kinase 2 is essential in maintenance of peripheral Foxp3+ regulatory T cells." in: Immunology, Vol. 154, Issue 2, pp. 309-321, (2018) (PubMed).

Phee, Au-Yeung, Pryshchep, OHagan, Fairbairn, Radu, Kosoff, Mollenauer, Cheng, Chernoff, Weiss: "Pak2 is required for actin cytoskeleton remodeling, TCR signaling, and normal thymocyte development and maturation." in: eLife, Vol. 3, pp. e02270, (2015) (PubMed).

Details zu PAK2

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Andere Bezeichnung: PAK2

Hintergrund: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58

Gen-ID: 5062

NCBI Accession: NP_002568

UniProt: Q13177

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Regulation of Lipid Metabolism by PPARalpha