TNF alpha Antikörper (PromoFluor 647 Premium)
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- Target Alle TNF alpha Antikörper anzeigen
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reaktivität
- Non-Human Primate
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser TNF alpha Antikörper ist konjugiert mit PromoFluor 647 Premium
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Applikation
- Flow Cytometry (FACS)
- Spezifität
- Native and recombinant TNF-α
- Kreuzreaktivität (Details)
- The monoclonal antibody reacts with human TNF-α and cross-react with TNF-α from non-human primates.
- Aufreinigung
- Purified from in vitro cultures by protein G affinity chromatography.
- Immunogen
- Recombinant human TNF-alpha
- Klon
- MT15B15
- Isotyp
- IgG1
- Top Product
- Discover our top product TNF alpha Primärantikörper
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha Reaktivität: Human, Maus, Ratte, Kaninchen, Pferd WB, ELISA, IHC (p), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha Reaktivität: Human, Ratte IHC, FACS, IF, StM Wirt: Maus Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha Reaktivität: Human, Schwein, Non-Human Primate FACS Wirt: Maus Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha Reaktivität: Maus WB Wirt: Kaninchen Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha Reaktivität: Maus WB, IHC, IP, ICC Wirt: Maus Monoclonal C1 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 71-234) antibodyTNF alpha Reaktivität: Rind (Kuh) WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) Wirt: Kaninchen Polyclonal unconjugated
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- Applikationshinweise
- Detection of human TNF-α producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of TNF-α by flow cytometry in viable PBMC from Rhesus macaque. Cells were stimulated for 4 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with MT15B15-PF647P (right panel). Matched isotype control antibody (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- in PBS with 0.2 % BSA and 0.09 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
- Haltbarkeit
- 18 months
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Andere Bezeichnung
- TNFA (TNF alpha Produkte)
- Gen-ID
- 7124
- Pathways
- NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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