TNF alpha Antikörper (PromoFluor-488 Premium)

Produktnummer ABIN6963905

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TNF alpha in FACS. Er zeigt eine Reaktivität gegenüber Non-Human Primate.

Kurzübersicht für TNF alpha Antikörper (PromoFluor-488 Premium) (ABIN6963905)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist konjugiert mit PromoFluor-488 Premium

Applikation: Flow Cytometry (FACS)

Klon: MT15B15

Produktdetails anti-TNF alpha Antikörper (PromoFluor-488 Premium)

Spezifität: Native and recombinant TNF-α

Kreuzreaktivität (Details): The monoclonal antibody reacts with human TNF-α and cross-react with TNF-α from non-human primates.

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human TNF-alpha

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Detection of TNF-α producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of TNF-α by flow cytometry in viable PBMC from Rhesus macaque. Cells were stimulated for 4 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with MT15B15-PF488P (right panel). Matched isotype control antibody (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNFA

Gen-ID: 7124

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome