Cyclin A Antikörper

Produktnummer ABIN6940983

Dieses Anti-Cyclin A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin A in IHC, IF, FACS und StM. Geeignet für Human, Maus, Rind (Kuh), Green Monkey und Nerz.

Kurzübersicht für Cyclin A Antikörper (ABIN6940983)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Rind (Kuh), Green Monkey, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: E67

Produktdetails anti-Cyclin A Antikörper

Spezifität: It recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Aufreinigung: Purified by Protein A/G

Immunogen: Full length bovine Cyclin A protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K-562 and HeLa cells. Human tonsil or lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: CCNA2

Molekulargewicht: 54kDa

Gen-ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA