MCM6 Antikörper (AA 228-368)

Produktnummer ABIN6940016

Der Maus Monoklonal anti-MCM6 Antikörper wird verwendet zum Nachweis von MCM6 in Proben von Human, Ratte, Meerschweinchen und Hamster. Er wurde validiert für IHC und WB.

Kurzübersicht für MCM6 Antikörper (AA 228-368) (ABIN6940016)

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reaktivität: Human, Ratte, Meerschweinchen, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Klon: MCM6-2999

Produktdetails anti-MCM6 Antikörper

Bindungsspezifität: AA 228-368

Verwendungszweck: MCM6 (Proliferation Marker) Antibody

Spezifität: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human skin or squamous cell carcinoma (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MCM6

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Andere Bezeichnung: MCM6

Hintergrund:

DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM

A-431, Human skin or squamous cell carcinoma (IHC). Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney or Guinea pig Kidney

Molekulargewicht: 105kDa

Gen-ID: 4175, 444118

UniProt: Q14566

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA