IGF1 Antikörper

Produktnummer ABIN6937486

Produktdetails anti-IGF1 Antikörper

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IGF1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Neutralization (Neut), Activity Assay (AcA), In vitro Assay (in vitro), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Kreuzreaktivität (Details): Human. Mouse. Rat. Rabbit.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified human IGF-1 protein

Klon: SPM406

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, purchase antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT), In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide)Optimal dilution for a specific application should be determined.

Positive_Control: Pancreas or brain. Breast, Thyroid or Colon Cancers, IGF-1 recombinant protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF1 (IGF1 Produkte)

Hintergrund: Insulin-like Growth Factor IA (IGF-IA), Insulin-like Growth Factor IB (IGF-IB), Mechano Growth Factor (MGF), Somatomedin-C,Insulin-like Growth Factor-1 (IGF-1)
Cellular localisation: Cytoplasmic (Secreted)

Molekulargewicht: ~7.6kDa

Gen-ID: 3479, 160562

UniProt: P05019

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation