CD14 Antikörper (AA 25-148)

Produktnummer ABIN6936603

Der Maus Monoklonal Anti-CD14-Antikörper wurde für FACS, WB, ISt und IHC (f) validiert. Er ist geeignet, CD14 in Proben von Human zu detektieren.

Kurzübersicht für CD14 Antikörper (AA 25-148) (ABIN6936603)

Target: CD14

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: LPSR-2385

Produktdetails anti-CD14 Antikörper

Bindungsspezifität: AA 25-148

Spezifität: Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Lipopolysaccharide receptor, LPS-R, LPSR, Mo2, Monocyte Differentiation Antigen 14, Myeloid cell-specific leucine-rich glycoprotein,CD14 (Monocyte / Macrophage Marker)
Cellular localisation: Cell Surface

Molekulargewicht: 55kDa

Gen-ID: 929, 163867

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades