Cyclin A Antikörper
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- Target Alle Cyclin A (CCNA2) Antikörper anzeigen
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
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Reaktivität
- Rind (Kuh)
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Cyclin A Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
- Kreuzreaktivität (Details)
- Human. Mouse. Cow. African Green Monkey. Mink (Mustela).
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Full length bovine Cyclin A protein
- Klon
- E67
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product CCNA2 Primärantikörper
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K-562 and HeLa cells. Human tonsil or lymph node.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
- Andere Bezeichnung
- CCNA2 (CCNA2 Produkte)
- Synonyme
- CCN1 antikoerper, CCNA antikoerper, AA408589 antikoerper, Ccn-1 antikoerper, Ccn1 antikoerper, Ccna antikoerper, CycA2 antikoerper, Cyca antikoerper, cb671 antikoerper, chunp6924 antikoerper, wu:fi36f03 antikoerper, CYCA antikoerper, Cyclin-A2 antikoerper, ccna2 antikoerper, ccn1 antikoerper, ccna antikoerper, ccna1 antikoerper, Cyclin-A antikoerper, cyclinA antikoerper, cyclin A2 antikoerper, cyclin A2 S homeolog antikoerper, cyclin A2 L homeolog antikoerper, CCNA2 antikoerper, Ccna2 antikoerper, ccna2 antikoerper, ccna2.S antikoerper, ccna2.L antikoerper
- Hintergrund
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CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2,Cyclin A2 (S- & G2-phase Cyclin)
Cellular localisation: Predominantly nuclear - Molekulargewicht
- 54kDa
- Gen-ID
- 890, 58974
- UniProt
- P20248
- Pathways
- PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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