PPP3CA Antikörper

Produktnummer ABIN6936568

Der Maus Monoklonal anti-PPP3CA Antikörper (Klon CALNA-2353) (ABIN6936568) detektiert spezifisch PPP3CA in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PPP3CA Antikörper (ABIN6936568)

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PPP3CA Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: CALNA-2353

Produktdetails anti-PPP3CA Antikörper

Verwendungszweck: Calcineurin A / PPP3CA Antibody

Spezifität: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Calcineurin A protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PPP3CA

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Andere Bezeichnung: Calcineurin A / PPP3CA

Hintergrund:

Calcineurin A alpha, Calcineurin A1, Calcineurin A, Calmodulin-dependent calcineurin A subunit alpha isoform, CALN, CALNA1, CAM-PRP catalytic subunit, CCN1, CNA alpha, CNA1, Ppp3ca, Protein phosphatase 2B catalytic subunit (PPP2B), Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform (PPP3CA)

HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.

Molekulargewicht: 62kDa

Gen-ID: 5530, 435512

UniProt: Q08209

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling