MAPK14 Antikörper (Mitogen-Activated Protein Kinase 14) (pThr180)

Details for Product anti-MAPK14 Antibody No. ABIN678668
Target
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • CRK1
  • Csbp1
  • Csbp2
  • Exip
  • Hog
  • Prkm14
  • Prkm15
  • Sapk2A
  • p38Hog
  • p38alpha
  • p38b
  • zp38b
  • MAPK14
  • 186F5S
  • BG:DS00797.3
  • CG7393
  • D-p38
  • D-p38 MAPK
  • D-p38b
  • Dm p38b
  • Dmel\\CG7393
  • Dmp38b
  • Dp38
  • Dp38b
  • ESTS:186F5S
  • Mpk34C
  • anon-sts23
  • dp38b
  • p38 MAPK
  • p38 beta
  • p38B
  • p38Kb
  • p38beta
  • Crk1
  • p38-alpha
  • p38MAPK
  • p38a
  • csbp
  • mapk14a
  • mxi2
  • sapk2
  • sapk2a
  • AP22.98
  • AP22_98
  • ATMPK14
  • mitogen-activated protein kinase 14
  • SAPK2a
  • MAP kinase 14A
  • MAP kinase p38a
  • MAPK 14A
  • fk28c03
  • hm:zeh1243
  • wu:fk28c03
  • zp38a
  • P38C-CRK
  • mitogen-activated protein kinase 14
  • mitogen activated protein kinase 14
  • mitogen-activated protein kinase 14b
  • p38b MAP kinase
  • mitogen-activated protein kinase 14 S homeolog
  • mitogen-activated protein kinase 14a
  • CRK proto-oncogene, adaptor protein
  • MAPK14
  • Mapk14
  • mapk14b
  • p38b
  • mapk14.S
  • MPK14
  • mapk14a
  • CRK
Bindungsspezifität
pThr180, pTyr182, AA 160-200
48
40
19
15
15
14
13
12
12
11
8
8
8
7
6
6
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Ziege, Human, Maus, Ratte
334
232
209
56
42
40
39
35
34
34
28
28
17
14
6
3
3
2
1
1
1
1
Wirt
Kaninchen
266
69
2
2
1
Klonalität
Polyklonal
Konjugat
Dieser MAPK14 Antikörper ist unkonjugiert
22
14
11
9
9
8
8
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
270
151
136
91
68
43
27
27
26
24
15
6
6
5
5
2
2
1
1
1
1
1
1
Optionen
Immunogen KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)
Isotyp IgG
Reinigung Purified by Protein A.
Target
Andere Bezeichnung P38 Mapk (MAPK14 Antibody Abstract)
Hintergrund

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Subcellular location: Cytoplasm, Nucleus

Synonyms: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, MAPK14

Gen-ID 1432
UniProt Q16539
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibo...
Image no. 2 for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Images provided by the Independent Validation Program (badge 29798).Lane one: HeLa ce...
Image no. 3 for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Images provided the Independent Validation Program (badge number 029662)Formalin-fixe...
Produkt verwendet in: Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." in: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, 2016 (PubMed).

Li, Yu, Liu, Zhu, Hua: "Expression of miR-34c in response to overexpression of Boule and Stra8 in dairy goat male germ line stem cells (mGSCs)." in: Cell biochemistry and function, Vol. 31, Issue 4, pp. 281-8, 2013 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Goat).

Wang, Sun, Liu, Yu, Li, Zeng, Chen, Jia: "Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration." in: FEMS immunology and medical microbiology, Vol. 63, Issue 1, pp. 82-92, 2012 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB)

'Independent Validation' Siegel
Chargennummer 140106
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human placenta tissue
Negativkontrolle Human tonsil tissue (no literature evidence of phospho-p38 MAPK in tonsil)
Bewertung Signal is detected in positive control tissue and not in negative control tissue.
Primärantikörper
  • Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182)
  • Catalog number: ABIN678668
  • Lot number: 140106
Isotypkontrolle
  • Antibody: Rabbit IgG isotype control
  • Lot number: C11245
Sekundärantikörper
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Lot number: D07640BA
Kontrollen
  • Positive control: Human placenta tissue stained with antibody
  • Negative control: Human tonsil tissue stained with antibody
  • Isotype control: Human placenta tissue stained with isotype control
  • Secondary only control: Human placenta tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 1: Human placenta tissue stained with anti-phospho-MAPK14 (brown) and counters...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 2: Human tonsil tissue stained with anti-phospho-MAPK14 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 3: Human placenta tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 4: Human placenta tissue stained with secondary antibody only (brown) and coun...