SIN3A Antikörper (N-Term)

Produktnummer ABIN6741541

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SIN3A in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Pferd, Hund, Schwein, Fledermaus, Hamster und Affe.

Kurzübersicht für SIN3A Antikörper (N-Term) (ABIN6741541)

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Hund, Schwein, Fledermaus, Hamster, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIN3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SIN3A Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human SIN3A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse (100%) Rabbit, Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SIN3A (Q96ST3, NP_056292). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%), Rabbit, Opossum, Guinea pig (92%), Zebra finch, Platypus (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SIN3A

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Andere Bezeichnung: SIN3A

Hintergrund: Name/Gene ID: SIN3A

Synonyms: SIN3A, DKFZP434K2235, KIAA0700

Gen-ID: 25942

NCBI Accession: NP_056292

UniProt: Q96ST3

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha