SIN3A Antikörper (N-Term)

Produktnummer ABIN2668357

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-SIN3A Antikörper wird verwendet zum Nachweis von SIN3A in Proben von Human und Maus. Er wurde validiert für WB und ChIP.

Kurzübersicht für SIN3A Antikörper (N-Term) (ABIN2668357)

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIN3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-SIN3A Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: SIN3A antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.

Anwendungsinformationen

Applikationshinweise: ChIP: 5 - 10 µL per ChIP WB*: 1:1,000 - 1:5,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-SIN3A Antikörper (ABIN2668357)

Chalei, Sansom, Kong, Lee, Montiel, Vance, Ponting: "The long non-coding RNA Dali is an epigenetic regulator of neural differentiation." in: eLife, Vol. 3, pp. e04530, (2014) (PubMed).

Details zu SIN3A

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Andere Bezeichnung: SIN3A

Hintergrund: SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.

Molekulargewicht: 155 kDa

Gen-ID: 25942

NCBI Accession: NP_001138829

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha