Cadherin 4 Antikörper (AA 458-641)

Produktnummer ABIN6719392

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cadherin 4 in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Cadherin 4 Antikörper (AA 458-641) (ABIN6719392)

Target: Cadherin 4 (CDH4)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cadherin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Cadherin 4 Antikörper

Bindungsspezifität: AA 458-641

Verwendungszweck: Anti-R-Cadherin-4 CDH4-Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-R-Cadherin-4 CDH4-Antibody Picoband® (ABIN6719392). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human R Cadherin/CDH4 recombinant protein (Position: K458-D641).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Chalmers, I. J., Hofler, H., Atkinson, M. J. Mapping of a cadherin gene cluster to a region of chromosome 5 subject to frequent allelic loss in carcinoma. Genomics 57: 160-163, 1999. 2. Suzuki, S., Sano, K., Tanihara, H. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul. 2: 261-270, 1991. 3. Tanihara, H., Sano, K., Heimark, R. L., St. John, T., Suzuki, S. Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin. Cell Adhes. Commun. 2: 15-26, 1994.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Cadherin 4

Target: Cadherin 4 (CDH4)

Andere Bezeichnung: CDH4

Hintergrund:

Synonyms: Cadherin-4, Retinal cadherin, R-CAD, R-cadherin, CDH4

Tissue Specificity: Expressed mainly in brain but also found in other tissues.

Background: Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 20q13.33. This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 125 kDa

Gen-ID: 1002

UniProt: P55283

Pathways: Cell-Cell Junction Organization, Regulation of Cell Size