TLR4 Antikörper

Produktnummer ABIN6657874

Hersteller Produkt- Nr.: 200-501-i26

Der Ratte Monoklonal Anti-TLR4-Antikörper wurde für WB, FACS, IP und IF validiert. Er ist geeignet, TLR4 in Proben von Maus zu detektieren.

Kurzübersicht für TLR4 Antikörper (ABIN6657874)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TLR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: MTS510

Produktdetails anti-TLR4 Antikörper

Hersteller Produkt- Nr.: 200-501-i26

Hersteller: Rockland

Verwendungszweck: TLR4 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR4 from mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-TLR4 Antibody was purified by Protein G chromatography.

Immunogen: TLR4 Antibody was produced in rats prepared by repeated immunizations with the Ba/F3 cell line expressing mouse protein TLR4.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 2-5 μg/10^6 cells

Western_Blot_Dilution: 1:500-1:1000

Kommentare:

Anti-TLR4 Antibody has been tested in ICC/IF, Flow (CS), and IP. Expect a band approximately 95kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4

Hintergrund:

Synonyms: hToll, Toll-like receptor 4, CD284

Background: Anti-TLR4 CD284 Antibody detects human TLR4. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa. Anti-TLR4 CD284 Antibody is ideal for invedtigators invovled in cytokines and growth factor research.

Gene Name: TLR4

Gen-ID: 21898

NCBI Accession: NP_067272

UniProt: Q9QUK6

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine