CD11b Antikörper (PE)

Produktnummer ABIN6657853

Hersteller Produkt- Nr.: 200-508-n79

Der Ratte Monoklonal Anti-CD11b-Antikörper wurde für FACS, IHC, IP, IF, FM und MA validiert. Er ist geeignet, CD11b in Proben von Maus zu detektieren.

Kurzübersicht für CD11b Antikörper (PE) (ABIN6657853)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Klon: M1-70

Produktdetails anti-CD11b Antikörper (PE)

Hersteller Produkt- Nr.: 200-508-n79

Hersteller: Rockland

Verwendungszweck: CD11b Phycoerythrin Antibody

Kreuzreaktivität (Details): Cross reactivity Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine).

Aufreinigung: Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b.

Sterilität: Sterile filtered

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotyp: IgG2b kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: 0.25 μg/10x6 cells

Kommentare:

Anti-CD11b is tested for FLOW and IF, and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b

Hintergrund:

Synonyms: CD11b

Background: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Gene Name: Itgam

UniProt: A1E2I0

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation